Primary antibody & Comparison of activating medicines

Primary Antibody	Clones		Products	DilutionA	Citrate Buffer	Tris-HCl+5% Urea	EDTA	TRS	Enzyme	Immunosaver	DilutionB
CD1a	mono	O10	Immunotech	stock						●	stock
CD2	mono	AB75	Novocastra	40-80			●			●	50
CD3	poly	Rabbit	DAKO	50-100	●			●		●	500
CD4	mono	1F6	Novocastra	20-40	●pH7		●			●	25
CD5	mono	4C7	Novocastra	25-50	●		●			●	200
CD7	mono	CD7-272	Novocastra	50-100	●					●	50
CD8	mono	C8/144B	DAKO	25-50				●		●	500
CD10	mono	56C6	Novocastra	80	●					●	50
CD15	mono	Leu-M1	Becton Dickinson	50						●	100
CD20	mono	L26	DAKO	50-100	●			●		●	10000
CD21	mono	2G9	Novocastra	10-20	●					●	50
CD23	mono	1B12	Novocastra	20-40	●p					●	200
CD25	mono	4C9	Novocastra	100-200	●p					●	100
CD30	mono	Ber-H2	DAKO	20-40	●		●	●		●	200
CD34	mono	NU-4A1	Nichirei	20-100						●	200
CD35	mono	RLB25	Novocastra	40-80			●			●	50
CD38	mono	SPC32	Novocastra	50-100	●					●	1000
CD43	mono	MT-1	Bio-Science	5-10				●		●	200
CD45	mono	2B11+PD7/26	DAKO	50-200	●					●	2000
CD45RA	mono	MB-1	Seikagaku kougyou	5-10						●	500
CD45RO	mono	UCHL-1	DAKO	50-75	●			●		●	700
CD56	mono	1B6	Novocastra	50-100	●p					●	100
CD57	mono	Leu-7	Becton Dickinson	50						●	100
CD68	mono	KP-1	DAKO	50-100	●					●	13000
CD68	mono	PGM-1	DAKO	50-100	●					●	500
CD74	mono	LN2	Nichirei	stock						●	10
CD79a	mono	JCB1117	DAKO	25-50	●A			●		●	500
CD99	mono	12E7	DAKO	50-75						●	1000
CD117	poly	Rabbit	DAKO	50						●	300
Keratin, wide	mono	Z622	DAKO	250-350					●	●	10000
CAM5.2	mono		Becton Dickinson	stock					●	●	50
CK7	mono	OV-TL 12/30	DAKO	25-50	●M			●		●	1000
CK20	mono	Ks20.8	DAKO	20-50	●			●		●	400
HMW	mono	34βE12	DAKO	50				●		●	50
CA125	mono	M11	DAKO	20	●					●	100
CyclinD1	mono	P2D11F11	Novocastra	20-50			●		●	●	30
c-erb B2	poly		Nichilei	stock	●AM					●	200
GCDFP-15	mono	23A3	Novocastra	20-40	●P					●	1000
ER	mono	1D5	DAKO	50-100				●Highi pH		●	30
PgR	mono	PgR636	DAKO	50	●			●		●	200
Ki-67	mono	MIB-1	DAKO	50	●		●	●		●	1000
P53	poly	Rabbit	Novocastra	200-1000	●					●	15000
P63	mono	4A4	DAKO	25-50		●	●			●	50
P80	mono	5A4	Novocastra	100	●p					●	200
PCNA	mono	PC10	DAKO	50-100	●					●	10000
IgA	poly	Rabbit	DAKO	1000-2000					●	●	10000
IgG	poly	Rabbit	MBL	50000					●	●	500000
IgM	poly	Rabbit	DAKO	200-400					●	●	50000
κchain	poly	Rabbit	DAKO	5000-20000					●	●	300000
λchain	poly	Rabbit	DAKO	10000-40000					●	●	200000
Melan-A	mono	A103	DAKO	25-50	●			●		●	30
MiTF	mono	Ab-3(5+D5)	NeoMarkers							●	30
Hepatocyte	mono	OCH1E5	DAKO	25-50	●					●	100
SP-A	mono	PE-10	DAKO	50-100						●	500
TTF-1	mono	8G7G311	DAKO	100-200	●					●	500
TdT	mono	SEN28	Novocastra	50-100	●					●	50
Factor VIII	mono	F8/86	DAKO	2000	●			●		●	4000
bcl-2	mono	123	DAKO	50-100	●A		●A			●	50
Granzymeβ	mono	11F1	Novocastra	40-80	●p					●	200
TIA-1	mono	2G9	Immunotech	100						●	200
LMP-1	mono	CS1-4	DAKO	25-50	●		●	●		●	1000
Calretinine	poly	Rabbit	Zymed	stock	●		●			●	stock
D2-40	mono	D2-40	Signet	40-60	●					●	stock
PAR-4	poly	Goat	Santa Cruz	50						●	50

TRS:Target Retrieval Solution A:Autoclave M:Microwave P:Pressure cooker

The dilution B data of Immunosaver is a condition when staining it with Ventana NX system NX-IHC/20 of the VENTANA Co.Ltd